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5S RNA‐Protein complex is involved in ribosomal subunit association
Author(s) -
Metspalu Ene,
Ustav Mart,
Villems Richard
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80131-8
Subject(s) - transfer rna , rnase p , ribosomal rna , protein subunit , eukaryotic large ribosomal subunit , rna , 5s ribosomal rna , ribonuclease , rnase mrp , ribosomal protein , microbiology and biotechnology , ribosome , biochemistry , biology , chemistry , gene
The immobilized tRNA‐50 S ribosomal subunit protein (TP50) complex binds the smaller ribosomal subunit. We constructed tRNA · TP50·5 S [ 32 P] RNA and tRNA·TP50·t [ 32 P] RNA complexes and investigated the accessibility of the 32 P‐labelled tRNAs to ribonuclease T 1 . It was found that in this complex both 5 S RNA and tRNA are attacked by T 1 RNase. In sharp contrast, the addition of 30 S subunit protects 5 S RNA as well as tRNA from degradation. We suggest that 5 S RNA‐TP50 complex is exposed to the ribosomal interface and is involved in subunit interaction.