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Mode of action of bottromycin A 2 : Effect of bottromycin A 2 on polysomes
Author(s) -
Otaka Tadahiko,
Kaji Akira
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80118-5
Subject(s) - polysome , puromycin , polynucleotide , ribosome , protein biosynthesis , translation (biology) , elongation , chemistry , biophysics , peptide , biochemistry , biology , rna , messenger rna , materials science , ultimate tensile strength , metallurgy , gene
When bottromycin A 2 was added to an in vitro protein synthesis system carried out by naturally occurring polysomes, it inhibited protein synthesis effectively. Examination of the 3 steps of peptide chain elongation revealed that the binding of aminoacyl‐tRNA to the polyribosomes was inhibited by bottromycin A 2 . In contrast, we concluded that the peptide bond formation and the translocation steps in this system were not inhibited by bottromycin A 2 on the basis of the following observation: (1) the break‐down of polysomes, which is dependent of EFG, puromycin and RR (ribosome releasing) factor, was insensitive to bottromycin A 2 ; (2) the puromycin dependent release of polypeptide from polysomes, with or without EFG, was not inhibited by bottromycin A 2 . Thus bottromycin specifically interferes with proper functioning of the A sites of polysomes. This is consistent with results obtained using the model system with synthetic polynucleotides.