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Preparation and neutralization characteristics of an antibody to the factor inducing differentiation of mouse myeloid leukemic cells
Author(s) -
Tomida M.,
Yamamoto-Yamaguchi Y.,
Hozumi M.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80087-8
Subject(s) - antiserum , in vitro , bone marrow , microbiology and biotechnology , colony stimulating factor , chemistry , inducer , antibody , myeloid , myeloid cells , biology , immunology , biochemistry , haematopoiesis , stem cell , gene
Mouse myeloid leukemic M1 cells can be induced to differentiate into macrophages and granulocytes in vitro by a protein inducer (D‐factor) in body fluids and conditioned media from various cells and tissues. Antiserum to D‐factor was prepared by immunizing a rabbit with D‐factor partially purified from conditioned medium of mouse L929 cells. At 1:20 dilution, the antiserum nearly completely suppressed the activities of various D‐factor preparations from mice and partially suppressed the activities of preparations from rats and hamsters. It did not cross‐react with factors stimulating colony formation of macrophages and granulocytes from normal bone marrow cells.