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Induction of ornithine decarboxylase by treatment of guinea pig lymphocytes with phospholipase C
Author(s) -
Kuramoto Ayako,
Otani Shuzo,
Matsui Isao,
Morisawa Seiji
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80076-3
Subject(s) - ornithine decarboxylase , cycloheximide , ornithine decarboxylase antizyme , phospholipase , biochemistry , guinea pig , phospholipase a , enzyme , biology , chemistry , phospholipase a2 , microbiology and biotechnology , protein biosynthesis , endocrinology
Treatment of guinea pig lymphocytes with Clostridium perfringens phospholipase C but not with Naja naja snake venom phospholipase A 2 increased ornithine decarboxylase activity. The increase in ornithine decarboxylase activity was suppressed by actinomycin D or cycloheximide, suggesting that de novo syntheses of RNA and protein are necessary for the increase in the enzyme activity. These results suggest that the activation of phospholipase C rather than that of phospholipase A 2 is responsible for induction of ornithine decarboxylase during lymphocyte transformation.