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The effects of glucocorticoids on thymidine kinase and nucleoside phosphotransferase during development of chicken embryo retina
Author(s) -
Tesoriere G.,
Tesoriere Luisa,
Giuliano M.,
Vento R.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80042-8
Subject(s) - embryo , thymidine kinase , phosphotransferase , nucleoside , thymidine , biology , hatching , endocrinology , kinase , retina , glucocorticoid , medicine , embryogenesis , biochemistry , enzyme , dna , microbiology and biotechnology , immunology , virus , zoology , herpes simplex virus , neuroscience
Thymidine kinase in chick embryo retina reaches its highest values on the 8–10th day of development, then declines reaching the lowest value at hatching. The rate of DNA synthesis essentially follows this activity while, in contrast, nucleoside phosphotransferase increases progressively during development. Glucocorticoids at 5 × 10 −6 M lower the level of thymidine kinase in isolated retinas of chick embryo. The most effective steroid was hydrocortisone. The effect was observed in retinas from 8–18‐day‐old chick embryo and, except on the 18th day, was always of the same magnitude. We suggest that a glucocorticoid can be the natural factor responsible for the marked fall in thymidine kinase during development. Brief periods of exposure to steriods increase nucleoside phosphotransferase activity in isolated chick embryo retinas. When the exposure was longer than 3 h this activity was also clearly decreased. We conclude that other factors are responsible for the natural increment which occurs for this activity during development.