Premium
pH‐jump‐induced phosphorylation of ADP in the cyanobacterium Anacystis nidulans
Author(s) -
Muchl Robert,
Peschek Günter A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80031-3
Subject(s) - chemistry , thylakoid , intracellular , chemiosmosis , biophysics , cyanobacteria , cyanide , membrane , extracellular , atp synthase , intracellular ph , membrane potential , cytoplasm , carbon monoxide , biochemistry , inorganic chemistry , chloroplast , bacteria , biology , catalysis , enzyme , genetics , gene
The low ATP levels in dark anaerobic cells of the cyanobacterium Anacystis nidulans more than doubled within 5 s after rapid addition of HCl shifting external pH from 9.0 to 4.5. Steady‐state levels of ATP and intracellular pH remained constant at 0.95 ± 0.15 nmol/mg dry weight and 6.9 ± 0.3, respectively. ΔpH‐induced ATP synthesis was inhibited by dicyclohexylcarbodiimide and carbonyl cyanide m ‐chlorophenylhydrazone but not by carbon monoxide. According to our results the cytoplasmic membrane of A. nidulans has to be regarded as an energy‐transducing membrane bioenergetically similar to the thylakoid membrane.