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Study of the binding of jatrophone to Escherichia coli s‐ribonucleic acid
Author(s) -
D'Alagni Maria,
De Petris Marco,
Battista Marini-Bettolo Giovanni,
Temussi Piero Andrea
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80017-9
Subject(s) - polynucleotide , escherichia coli , multiplicity (mathematics) , nucleotide , chemistry , transfer rna , molecule , dna , rna , crystallography , stereochemistry , biochemistry , organic chemistry , mathematics , gene , mathematical analysis
The interaction of jatrophone with sRNA with sRNA from Escherichia coli has been investigated through UV, CD, and 1 H NMR measurements. The results obtained show that the interaction with jatrophone increases the stability of the polynucleotide. It appears that the optical properties of jatrophone depend upon the jatrophone/nucleotide ratio. The observed behaviour can only be explained by the existence of different types of interaction between jatrophone and sRNA. Even for a jatrophone/nucleotide ratio as low as 0.17 the 1 H NMR spectra show a multiplicity of resonances that can only be explained by the simultaneous existence of two different binding modes involving the jatrophone molecules.