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Resolution and reconstitution of the rec BC deoxyribonuclease of Escherichia coli
Author(s) -
Banfalvi G.,
Csuzi S.,
Antoni F.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80012-x
Subject(s) - nuclease , deoxyribonuclease , dna , escherichia coli , atpase , duplex (building) , enzyme , chemistry , biochemistry , deoxyribonucleases , microbiology and biotechnology , deoxyribonuclease i , biology , base sequence , gene
The inactivation of rec BC nuclease activity and simultaneously the separation of 3 DNA‐dependent ATPases and an ATP‐independent DNases specific for single‐stranded DNA have been observed after DEAE‐cellulose chromatography of cell extracts from Escherichia coli . Two of the ATPases catalyze the strand separation of duplex DNA. Reconstitution of ATP‐dependent DNase activity has been carried out by the combination of the separation enzymes.