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Effect of ribonuclease H from chick embryo on the covalent‐linked poly(A)—poly(dA) complementary to poly(dT) template
Author(s) -
Sawai Yasuko,
Kitahara Namiko,
Tsukada Kinji
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81340-9
Subject(s) - covalent bond , ribonuclease , embryo , chemistry , microbiology and biotechnology , biochemistry , biology , rna , organic chemistry , gene
In vitro poly(dA) synthesis on poly(dT) template can be initiated by poly(A) primer. Poly(A) chains are covalently extended by DNA polymerase. The reaction product consists of poly(dA) chain with poly(A) at their 5′‐ends, hydrogen bonded to the template poly(dT). The primer poly(A) is linked to the product poly(dA) via a 3′ :5′‐phosphodiester bond, and can be specifically removed by ribonuclease H from chick embryos, leaving a 5′‐phosphate end of poly(dA). Poly‐ or oligoriboadenylate longer than the (pA) 5 could serve as a priming activity to synthesize poly(A) covalently linked to poly(dA).