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A protein essential for recovering oxygen evolution in cholate‐treated chloroplasts
Author(s) -
Toyoshima Yoshinori,
Fukutaka Eitaro
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81339-2
Subject(s) - thylakoid , chloroplast , chemistry , spinach , biochemistry , chromatography , glycerol , membrane , oxygen , organic chemistry , gene
A novel method for the reconstitution of oxygen evolution in cholate‐extracted spinach thylakoid membranes was established and a protein essential for the reconstitution was purified from cholate extracts. Purification of the protein was accomplished by chromatography on a DEAE‐Sephacel column. This protein ( M r 17 000) was reinserted into vesicular membranes reconstituted from cholate‐extracted thylakoids in the presence of 25% glycerol to reactivate oxygen evolution.