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In vivo actions of protein phosphatase inhibitor‐2 in Xenopus oocytes
Author(s) -
Foulkes J.Gordon,
Maller James L.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81325-2
Subject(s) - dephosphorylation , xenopus , phosphatase , protein phosphatase 1 , oocyte , phosphorylation , in vivo , microbiology and biotechnology , meiosis , chemistry , biology , biochemistry , embryo , genetics , gene
Meiotic maturation of amphibian oocytes induced by progesterone is known to be regulated by protein phosphorylation. To investigate a possible role for protein phosphatase‐1 in this process, the effect of phosphatase inhibitor‐2 was determined on the in vivo rate of dephosphorylation of phosphorylase a and on the rate of oocyte maturation. Dephosphorylation of microinjected phosphorylase a was inhibited up to 40% in the presence of inhibitor‐2, with half‐maximal inhibition at an intracellular concentration of 0.6 μM. Inhibitor‐2 also caused over a 3‐fold increase in the half‐time for maturation, suggesting a possible role for protein phosphatase‐1 in the regulation of meiosis.