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Identification of a M r 58 000 glycoprotein subunit of the opiate receptor
Author(s) -
Klee Werner A.,
Simonds William F.,
Sweat Floyd W.,
Burke Terrence R.,
Jacobson Arthur E.,
Rice Kenner C.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81318-5
Subject(s) - wheat germ agglutinin , opiate , chemistry , dextrorphan , receptor , isothiocyanate , protein subunit , biochemistry , microbiology and biotechnology , biology , gene , lectin
A M r 58 000 subunit of the opiate receptor has been identified using tritiated fentanyl isothiocyanate, a potent opiate alkylating reagent with specificity for the δ‐opiate receptor subclass. The subunit is alkylated in the presence of dextrorphan but not levorphanol. The specifically labelled protein was retained on columns of immobilized wheat germ agglutinin and is therefore presumably a glycoprotein. Partial purification of the M r 58 000 opiate receptor subunit from neuroblastoma × glioma NG108‐15 hybrid cell membranes is described.