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Valinomycin can depolarize mitochondria in intact lymphocytes without increasing plasma membrane potassium fluxes
Author(s) -
Felber Suzanne M.,
Brand Martin D.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81317-3
Subject(s) - valinomycin , ionophore , membrane potential , depolarization , potassium , membrane , chemistry , biophysics , mitochondrion , plasma , nigericin , biochemistry , biology , organic chemistry , physics , quantum mechanics
We have simultaneously measured [ 3 H]methyltriphenylphosphonium + and 86 Rb + distribution across the mitochondrial and plasma membranes of pig mesenteric lymphocytes. At low concentrations the K + ionophore valinomycin depolarizes the mitochondria but does not increase Rb + fluxes across the plasma membrane. At high concentrations of valinomycin the plasma membrane becomes permeable to Rb + and both TPMP + and Rb + distribution give a measure of the K + diffusion potential.