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The effect of estrogen on the concentration of ovalbumin gene sequence in the 2 M NaCl residual fraction of oviduct chromatin
Author(s) -
Mirell Carol J.,
Bekhor Isaac
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81316-1
Subject(s) - ovalbumin , oviduct , chromatin , estrogen , fraction (chemistry) , chemistry , residual , sequence (biology) , gene , andrology , biology , endocrinology , chromatography , biochemistry , genetics , medicine , mathematics , antigen , algorithm
Oviduct chromatin was isolated from both estrogenized and non‐estrogenized hens. Extraction of the chromatin with 2 M NaCl removed a majority of the proteins, and the resulting DNA was then separated into two components: (1) a major fraction which was virtually protein‐free; and (2) a minor fraction which was complexed with proteins. It was found that the DNA fraction that is complexed with proteins contained ovalbumin gene sequences and that the concentration of these sequences could be boosted by estrogen‐treatment.