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Nde I: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5′‐CATATG‐3′ sequences
Author(s) -
Watson Robert J.,
Schildkraut Ira,
Qiang B.-Q.,
Martin Stan M.,
Visentin Louis P.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81315-x
Subject(s) - watson , library science , research council , biology , political science , philosophy , government (linguistics) , linguistics , natural language processing , computer science
Type II restriction endonucleases recognize and cleave near specific DNA sequences, usually 4-6 basepair palindromes. They are of fundamental importance as tools for the recombinant DNA technology as they provide the selective cleavages required for the analysis and restructuring of DNA in vitro. The flexibility of these techniques is proportional to the number of DNA sequences which can be cleaved by available enzymes. Here, we describe the isolation and characterization of a restriction enzyme from Neisseria denitrificans with a new recognition sequence, 5’-CATATG-3’.