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A* protein of bacteriophage φX174 carries an oligonucleotide which it can transfer to the 3−OH of a DNA chain
Author(s) -
van Mansfeld A.D.M.,
van Teeffelen H.A.A.M.,
Zandberg J.,
Baas P.D.,
Jansz H.S.,
Veeneman G.H.,
van Boom J.H.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81313-6
Subject(s) - oligonucleotide , dna , microbiology and biotechnology , bacteriophage , cleavage (geology) , biology , gene , endonuclease , biochemistry , chemistry , escherichia coli , paleontology , fracture (geology)
The bacteriophage φX174 gene A encodes two proteins: gene A protein and A * protein. Purified A * protein acts as a single‐stranded, DNA‐specific endonuclease which remains covalently attached to the 5′‐end of the cleavage site. Incubation of A * protein with the synthetic heptamer CAACTTG or with oligonucleotides which yield this heptamer after cleavage with the A * protein yields oligonucleotides with the sequences CAACTTGAG, CAACTTGAGG and CAACTTGAGGA. This indicates that A * protein carries an oligonucleotide with the sequence ‐AG, AGG or ‐AGGA. The oligonucleotide can be transferred to the 3′‐end of the heptamer CAACTTG. This suggests that A * protein reacts with a specific DNA sequence in the infected cell.