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Affinity chromatography of Vicia graminea (blood group N) lectin using concanavalin A—Sepharose
Author(s) -
Prigent M.J.,
Lombart C.,
Asseraf A.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81309-4
Subject(s) - concanavalin a , affinity chromatography , lectin , size exclusion chromatography , chromatography , sepharose , polyacrylamide gel electrophoresis , chemistry , yield (engineering) , biochemistry , enzyme , in vitro , materials science , metallurgy
The lectin extracted from Vicia graminea seeds has been purified by conventional techniques but such procedures did not give a satisfactory yield. We describe a new purification which involves 3 steps after obtention of the crude extract. The first step is based on affinity chromatography on con A—Sepharose. Further purification steps were performed on DEAE‐Sephacel chromatography and ultrogel AcA 44 gel filtration. The homogeneity of the lectin was demonstrated by polyacrylamide gel electrophoresis. Purification of the lectin by this new method was less time consuming, the yield was higher and the specific activity increased.