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Uridine as the only alternative to pyrimidine de novo synthesis in rat T lymphocytes
Author(s) -
Bismuth Georges,
Thuillier Laure,
Perig Jean-Louis,
Cartier Pierre H.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81259-3
Subject(s) - cytidine , uridine , uracil , thymidine , dna synthesis , thymine , deoxyuridine , de novo synthesis , pyrimidine , cytosine , pyrimidine metabolism , microbiology and biotechnology , chemistry , biochemistry , deoxycytidine , biology , cell growth , dna , rna , purine , enzyme , genetics , chemotherapy , gemcitabine , gene
Concanavalin A‐induced proliferation of rat T‐lymphocytes is completely inhibited by 10 −5 M pyrazofurin, a potent inhibitor of pyrimidine de novo synthesis, as judged by cell viability and [ 3 H]thymidine incorporation. Proliferation is completely restored by 5 × 10 −5 M uridine. Cytidine, deoxycytidine, deoxyuridine and thymidine 10 × 10 −5 M each, fail to re‐establish proliferation but produce an isotropic dilution of [ 3 H]thymidine uptake in DNA. Bases (cytosine, uracil and thymine) neither restore proliferation nor induce isotopic dilution. The unexpected inability of cytidine to reverse de novo pyrimidine synthesis inhibition suggests a lack of cytidine deaminase activity in rat T‐lymphocytes. This is confirmed by a direct sensitive radioisotopic assay (<0.001 nmol.min −1 .10 −6 cells).