Premium
The effect of sodium channel activators on muscarinic receptors of neuroblastoma cells
Author(s) -
Milligan Graeme,
Strange Philip G.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81238-6
Subject(s) - muscarinic acetylcholine receptor , veratridine , chemistry , muscarinic acetylcholine receptor m5 , muscarinic acetylcholine receptor m1 , agonist , receptor , muscarinic acetylcholine receptor m3 , endocrinology , muscarinic acetylcholine receptor m2 , muscarinic acetylcholine receptor m4 , acetylcholine , incubation , muscarinic agonist , medicine , pharmacology , biophysics , biochemistry , sodium , sodium channel , biology , organic chemistry
Incubation of neuroblastoma N1E 115 cells with veratrine leads to an apparent reduction in the number of muscarinic acetylcholine receptors assayed by [ 3 H]scopolamine methyl chloride binding. No true down‐regulation of the receptors occurs but a component of veratrine with muscarinic receptor affinity, which is not veratridine, enters the intracellular water space during the incubation period and competes with [ 3 H]scopolamine methyl chloride for the muscarinic binding sites in subsequent ligand binding assays unless it is carefully washed away. Treatment of cells with the agonist carbamoylcholine does, however, lead to a true downregulation of muscarinic receptors.