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Bovine cardiac troponin subunits: binary complexes and reconstitution of whole troponin
Author(s) -
Byers David M.,
Kay Cyril M.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81232-5
Subject(s) - troponin , stokes radius , troponin i , troponin complex , size exclusion chromatography , troponin t , troponin c , chromatography , chemistry , protein subunit , biochemistry , cardiology , medicine , enzyme , myocardial infarction , gene
The reconstitution of bovine cardiac troponin from its subunits has been investigated using hydrodynamic techniques. Gel filtration (Sephacryl S‐300) and sedimentation velocity experiments indicate that troponin‐C and troponin‐I from a stable binary complex (1:1 mole ratio) with an apparent Stokes' radius of 36 Å (frictional ratio = 1.6). Troponin‐C and troponin‐T do not interact significantly while troponin‐I and troponin‐T undergo partial complex formation. The effect of subunit ratio on the reconstitution of whole troponin has been examined by SDS—polyacrylamide gel electrophoresis and gel filtration and the results suggest that native troponin contains the subunits in an equimolar ratio.