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A translational inhibitor activated in rabbit reticulocyte lysates under high pO 2
Author(s) -
Almış-Kanıgür Gönül,
Kan Beki,
Kospançalı Serpil,
Bermek Engin
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81223-4
Subject(s) - reticulocyte , hemin , chemistry , biochemistry , sephadex , glutathione , microbiology and biotechnology , enzyme , biology , heme , messenger rna , gene
An inhibitor of protein synthesis was activated under high oxygen partial pressure (pO 2 ) in hemin‐supplemented and glutathione disulfide‐free lysates from rabbit reticulocytes. This inhibitor shared some common features with other translational inhibitors from rabbit reticulocytes; that is, hemin‐controlled repressor, glutathione disulfide‐activated inhibitor and high pressure‐activated inhibitor. It caused biphasic kinetics of inhibition which could be potentiated by ATP. Its activation was prevented by cAMP or glucose 6‐phosphate. The high pO 2 ‐inhibitor could be partially purified from post‐ribosomal supernatant containing ribosomal salt wash by precipitation between 0–50% (NH 4 ) 2 SO 4 ‐saturation, Sephadex G‐100, and DEAE‐cellulose chromatography.