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The effect of Ca 2+ on the stability of chicken erythrocyte histone octamers
Author(s) -
Lindsey George G.,
Thompson Patricia,
Pretorius Linda,
Purves Langley R.,
von Holt Claus
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81116-2
Subject(s) - histone octamer , histone , chemistry , dissociation (chemistry) , chromatin , calcium , histone h2b , tyrosine , biophysics , nucleosome , fluorescence , fluorescence spectroscopy , biochemistry , dna , biology , organic chemistry , physics , quantum mechanics
Histones can be extracted from chicken erythrocyte chromatin with 2 M CaCl 2 10 mM Tris (pH 7.4). The core histones so extracted exist as H3–H4 tetramers and H2A–H2B dimers since calcium at >0.5 M result in dissociation of the histone octamer. Reconstitution of octamers occurs on removal of the Ca 2+ by dialysis. Although <0.5 M calcium do not result in octamer dissociation, perturbations in the structure can be detected by CD and tyrosine fluorescence spectroscopy.