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Rapid, transient methylation of four proteins in aggregative amoebae of Dictyostelium discoideum as a response to stimulation with cyclic AMP
Author(s) -
van Waarde Aren
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81114-9
Subject(s) - dictyostelium discoideum , methylation , chemotaxis , stimulation , dictyostelium , microbiology and biotechnology , extracellular , biology , chemistry , biochemistry , gene , receptor , endocrinology
In Dictyostelium discoideum , extracellular cAMP induces chemotaxis and cell aggregation. Suspensions of cAMP‐sensitive cells are shown to respond to a 10 −6 M cAMP‐pulse with increased methylation of 4 proteins with app. M r 110000, 46000, 28000 and 16000. The M r 110000 and 28000 proteins show a triphasic response with maxima 15, 60 and 150–180s after stimulation. The responses of the M r 46000 and 16000 proteins are monophasic, maxima being reached 3 and 15s after stimulation, respectively. Optimal responses of methylation are observed over 10 −7 −10 −6 M cAMP. The methylation reaction may be involved in the processing of the chemotactic signal.