Premium
Inhibition of electrogenic anion entry into rat liver mitochondria by N,N' ‐dicyclohexylcarbodiimide
Author(s) -
Warhurst Ian W.,
Dawson Alan P.,
Selwyn Michael J.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81110-1
Subject(s) - mitochondrion , chemistry , reagent , biochemistry , adipose tissue , brown adipose tissue , organic chemistry
The carboxyl group reagent dicyclohexylcarbodiimide inhibits the electrogenic entry of Cl − and NO 3 − into rat liver mitochondria at alkaline pH. The inhibition is time dependent and 50% inhibition is obtained by the addition of 3–4 nmol DCCD/mg protein. The blockage of the pH‐dependent anion‐conducting pore appears to be unrelated to the other known actions of DCCD on rat liver mitochondria but seems similar to its effect on the uncoupling protein of brown adipose tissue.