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Transmembrane potential variations accompanying the PMA‐triggered O − 2 and H 2 O 2 release by mouse peritoneal macrophages
Author(s) -
Lepoivre Michel,
Tenu Jean-Pierre,
Petit Jean-François
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81107-1
Subject(s) - depolarization , egta , stimulation , membrane , chemistry , membrane potential , macrophage , biophysics , microbiology and biotechnology , biology , biochemistry , calcium , in vitro , endocrinology , organic chemistry
Stimulation by PMA of Streptococci‐elicited macrophages induced a transient membrane depolarization preceding the onset of detectable O − 2 production. Mice‐resident peritoneal macrophages were unresponsive to PMA for both activities. The PMA‐triggered membrane depolarization seemed to be independent from O − 2 production because inhibition of membrane depolarization by EGTA had no effect on rates of O − 2 or H 2 O 2 release and rate of antimycin A insensitive O 2 uptake by Streptococci‐elicited macrophages. The portion of O 2 uptake recovered as O − 2 was found to be . The rate of O − 2 release was twice the rate of H 2 O 2 production (1.1 nmol H 2 O 2 .min − .10 6 macrophages −1 ).