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Quantification of the organophosphorus nerve agent soman by competitive inhibition enzyme immunoassay using monoclonal antibody
Author(s) -
Hunter Kenneth W.,
Lenz David E.,
Brimfield Alan A.,
Naylor Juliette A.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81091-0
Subject(s) - medicine , family medicine
The organophosphorus compound soman (methylphosphonofluoridic acid 1,2,2-trimethylpropyl ester) is a potent cholinesterase inhibitor [1,2] and a chemical warfare agent. The rapid identification and characterization of nerve agents such as soman would facilitate the diagnosis and treatment of poisoned humans, and provide a more useful means of environmental surveillance. Presently, soman is routinely identified by physicochemical techniques such as gas chromatography [3-51. Though sensitive and specific, these techniques are time consuming and require expensive equipment and highly trained operators. Here, we discuss the production of a mouse monoclonal antibody that binds to soman and further describe the use of this antibody in a competitive inhibition enzyme immunoassay (CIEIA) capable of quantifying levels of soman as low as 1.0 x 10v6M (200ppb). The specificity of this antibody is demonstrated by its ability to distinguish between soman and the structurally similar organophosphorus nerve agent sarin (methylphosphonofluoridic acid, l-isopropyl ester). hemocyanin from giant keyhole limpets (KLH) was purchased from Schwarz-Mann (Orangeberg NY). Soman and sarin (>95% pure) were obtained from Chemical Systems Labs. (Aberdeen Proving Ground MD). All other chemicals were reagent grade and obtained commercially.