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A photo‐CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins
Author(s) -
Moonen Chrit T.W.,
Hore Peter J.,
Müller Franz,
Kaptein Robert,
Mayhew Stephen G.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81090-9
Subject(s) - cidnp , flavodoxin , chemistry , tryptophan , tyrosine , nuclear magnetic resonance spectroscopy , methionine , clostridium , spectroscopy , stereochemistry , amino acid , biochemistry , bacteria , biology , physics , ferredoxin , enzyme , genetics , radical , quantum mechanics
Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo‐CIDNP techniques. Using time‐resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR‐spectra to tyrosine, tryptophan and methionine residues in the two proteins. The results show that Trp‐91 in M.elsdenii and Trp‐90 in Cl.MP flavodoxin are strongly immobilized and placed directly above the benzene subnucleus of the prosthetic group. The data further indicate that the active sites of the two flavodoxins are extremely similar.