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ESR study of the primary electron donor in highly 13 C‐enriched Chlorobium limicola f. thiosulfatophilum
Author(s) -
Wasielewski Michael R.,
Smith Ursula H.,
Norris James R.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81089-2
Subject(s) - bacteriochlorophyll , photosynthetic reaction centre , chemistry , delocalized electron , electron donor , electron paramagnetic resonance , spin (aerodynamics) , electron , bacteria , crystallography , nuclear magnetic resonance , photosynthesis , photochemistry , physics , electron transfer , biology , biochemistry , genetics , organic chemistry , quantum mechanics , thermodynamics , catalysis
The photo‐induced electron spin resonance signal of the primary donor P840 + in unit membrane fractions of the title photosynthetic bacterium was measured. Bacteria containing natural isotopic abundances and 82% 13 C‐enrichment were examined. Second moment analysis of the lineshape of the 13 C‐enriched P840 + signal shows that P840 + consists of two bacteriochlorophyll a ‐type macrocycles. The data thus obtained are independent of difficulties in interpreting either peak‐to‐peak linewidths or ENDOR data with respect to intermolecular spin delocalization.