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Evidence for erythrocyte membrane glycoproteins being carriers of blood‐group P 1 determinants
Author(s) -
Haselberger Christian G.,
Schenkel-Brunner Helmut
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81086-7
Subject(s) - pronase , glycoprotein , glycolipid , membrane , erythrocyte membrane , red blood cell , membrane glycoproteins , chemistry , biochemistry , trypsin , enzyme
The contribution of different membrane constituents to the bloodgroup P 1 activity of human erythrocytes was investigated. Pronase digestion of native red cell stroma or partition between butanol and water had no serologically detectable effect, whereas pronase‐treatment of previously butanol‐extracted membranes liberated virtually all blood‐group P 1 determinants from the ghosts. On Laemmli gels, all P 1 activity was found in the band 4.5 region. Thus it is concluded that, in addition to the well‐documented P 1 glycolipid, also membrane glycoproteins are carriers of blood‐group P 1 determinants.