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Histamine interaction on surface recognition sites of H 2 ‐type in parietal and non‐parietal cells isolated from the guinea pig stomach
Author(s) -
Gespach Christian,
Bouhours Danièle,
Bouhours Jean-François,
Rosselin Gabriel
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81077-6
Subject(s) - histamine , parietal cell , gastric mucosa , guinea pig , cimetidine , enterochromaffin like cell , medicine , stomach , pronase , diphenhydramine , chemistry , endocrinology , histamine receptor , posterior parietal cortex , antagonist , biology , receptor , biochemistry , trypsin , enzyme , neuroscience
In gastric cells isolated by pronase digestion from the guinea pig, histamine stimulated cAMP production in 3 fundic cell fractions ( EC 50 = 1.6–2 × 10 −4 M) enriched in parietal (94%), peptic (63%) and mucous cells (87%) as well as in antral cells ( EC 50 = 4 × 10 −4 M) that are devoid of parietal cells. Histamine stimulations were completely inhibited by the H 2 antagonist cimetidine ( K i = 0.27–0.57 × 10 −6 M) or by the H 1 antagonist diphenhydramine, but at 100‐times lower potency ( K i = 22–45.7 × 10 −6 M), indicating the presence of histamine H 2 receptors in parietal and nonparietal cells of the guinea pig gastric mucosa.