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The mandatory role of complement in the endotoxin‐induced synthesis of tissue thromboplastin in blood monocytes
Author(s) -
Østerud Bjarne,
Eskeland Trond
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81075-2
Subject(s) - thromboplastin , tissue factor , monocyte , chemistry , incubation , partial thromboplastin time , immunology , medicine , endocrinology , platelet , biochemistry , coagulation
Fres isolated blood cells recombined with normal heparinized plasma and then incubated with endotoxin, induced a 100‐fold increase in monocyte tissue thromboplastin synthesis. In contrast, recombination of these cells with heat inactivated plasma, cobra venom factor‐treated plasma, Ca 2+ ‐free plasma, or BioRex 70‐treated plasma (plasma free of Clq and D) before incubation with endotoxin, failed to induce monocyte synthesis of tissue thromboplastin. These results strongly support the hypothesis that complement is required for endotoxin stimulation of blood monocyte synthesis of tissue thromboplastin.