Premium
Folding and unfolding of the core particle DNA are processes faster than millisecond
Author(s) -
Erard M.,
Burggraf E.,
Pouyet J.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81070-3
Subject(s) - stopped flow , cuvette , chemistry , chromatin , biophysics , nucleosome , millisecond , dissociation (chemistry) , particle (ecology) , core (optical fiber) , dna , folding (dsp implementation) , linker dna , chemical physics , crystallography , physics , kinetics , optics , biochemistry , biology , ecology , electrical engineering , quantum mechanics , astronomy , reaction rate constant , engineering
Core particle DNA unfolding and refolding are followed by stopped‐flow circular dichroism technique. When core particles are dissociated in the stopped‐flow cuvette, the high CD deviation corresponding to the dissociated state is reached in the first millisecond, which means that the dissociation process is completed within the dead time of the apparatus which is ∼1 ms. The same conclusion can be drawn when core particles are reassociated, since the low CD value, typical of the associated state, is immediately reached. Similarly histone release from chromatin is a very fast process. We also include some points of discussion about core particle assembly process.