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DNA‐dependent ATPase B of FM3A cells
Author(s) -
Watanabe Yoshinari,
Nagata Kyosuke,
Tawaragi Yasunori,
Enomoto Takemi,
Hanaoka Fumio,
Yamada Masa-atsu
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81067-3
Subject(s) - dna , atpase , biochemistry , microbiology and biotechnology , dna polymerase , enzyme , glycerol , centrifugation , differential centrifugation , chemistry , exogenous dna , biology
One form of DNA‐dependent ATPase (DNA‐dependent ATPase B) has been purified from FM3A cells. In this report, we describe the association of DNA polymerase α activity with DNA‐dependent ATPase B through a series of purification steps and the final separation of the two enzymes by glycerol gradient centrifugation operated at a low salt concentration.