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Association of cap binding protein‐related polypeptides with cytoplasmic RNP particles of chick embryonic muscle
Author(s) -
Chakraborty Dipak,
Mukherjee Asok K.,
Sarkar Satyapriya,
Lee Kevin A.W.,
Darveau Andre,
Sonenberg Nahum
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81064-8
Subject(s) - polysome , cytoplasm , ribonucleoprotein , messenger rnp , biology , microbiology and biotechnology , translation (biology) , immunoprecipitation , biochemistry , peptide , messenger rna , chemistry , ribosome , rna , gene
Cap binding protein (CBP)‐related polypeptides were identified in different cytoplasmic RNP particles of embryonic chick muscles using monoclonal antibody to purified CBP. A single immunoreactive peptide ( M r 78000) was present in preparations of both free mRNP particles and a novel 10 S translation inhibitory RNP particle. In contrast, proteins isolated from these particles showed two new low‐ M r immunoreactive peptides ( M r 43000 and M r 29000). No CBP related protein could be detected in polysomal mRNP, although an immunoreactive M r 43000 CBP‐related protein was present in polysomes. The relevance of the association of different CBP‐related polypeptides with cytoplasmic RNP particles and polysomes are discussed.