Premium
Distribution of calmodulin within wheat leaf cells
Author(s) -
Muto Shoshi
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81032-6
Subject(s) - calmodulin , chloroplast , nad+ kinase , cytosol , biochemistry , enzyme , kinase , mitochondrion , chemistry , biology , microbiology and biotechnology , gene
Subcellular distribution of calmodulin within wheat leaf cells was measured by a enzymatic method (activation of calmodulin‐deficient NAD kinase from pea seedlings) or a radioimmunoassay. Most of cellular calmodulin was localized in cytosol fraction (89–93%), and remainders were in the mitochondrial (5–9%), the chloroplast (1–2%) and the microsomal (< 1%) fractions. Calmodulin concentration in the chloroplast was sufficient to saturate NAD kinase which was localized mostly in the chloroplast. Therefore, the light‐induced Ca 2+ transport in intact wheat chloroplasts (Muto, S. FEBS Lett. (1982) 139, 250–254] may play an important role in regulation of chloroplast NAD kinase activity.