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Non‐vectorial phosphorylation by the bacterial PEP‐dependent phosphotransferase system is an artifact of spheroplast and membrane vesicle preparation procedures
Author(s) -
Robillard G.T.,
Lageveen R.G.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)81028-4
Subject(s) - spheroplast , membrane , vesicle , population , phosphorylation , biophysics , chemistry , artifact (error) , biochemistry , biology , escherichia coli , neuroscience , sociology , gene , demography
These data demonstrate that the phenomenon of non‐vectorial phosphorylation [Saier, M.H. jr and Schmidt, M.R. (1981) J. Bacteriol. 145, 391–397] is an experimental artifact which arises during the conversion of whole cells to spheroplasts or membrane vesicles. It can be explained by a certain population of particles which are not properly sealed. There is no need to envoke two orientations of enzyme II in the membrane, one of which would have no physiological significance.