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Complexes of cytochrome P450 with metyrapone
Author(s) -
Mitani Fumiko,
Shephard Elizabeth A.,
Phillips Ian R.,
Rabin Brian R.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80829-6
Subject(s) - phenobarbital , metyrapone , cytochrome p450 , microsome , radioimmunoassay , chemistry , cytochrome , immunoprecipitation , biochemistry , enzyme , endocrinology , biology , gene
Phenobarbital‐inducible cytochrome P450 variants have a higher affinity (app. K s = 0.6 μM) for metyrapone than do non‐phenobarbital‐inducible variants (app. K s = 80 μM). The difference in affinity has enabled us to quantitate, by metyrapone‐induced difference spectral analysis, phenobarbital‐inducible cytochromes P450 in liver microsomal membranes. The result obtained by this simple, rapid technique compare well with those found by more sophisticated techniques such as radioimmunoassay and immunoprecipitation.