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Archaebacterial elongation factor Tu insensitive to pulvomycin and kirromycin
Author(s) -
Cammarano Piero,
Teichner Angela,
Chinali Gianni,
Londei Paola,
de Rosa Mario,
Gambacorta Agata,
Nicolaus Barbara
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80819-3
Subject(s) - escherichia coli , ef tu , biology , thermophile , biochemistry , bacteria , genetics , rna , transfer rna , gene
A spermine‐dependent, polyphenylalanine‐synthesizing cell‐free system having an optimum activity at 75–85°C, has been developed from the extremely thermoacidophilic archaebacterium Caldariella acidophila . The C. acidophila system is totally insensitive to the EF‐Tu targeted antibiotics pulvomycin (at 40°C) and kirromycin (at 47–72°C) contrary to control systems derived from both mesophilic ( Escherichia coli ) and thermoacidophilic ( Bacillus acidocaldarius ) eubacteria. The archaebacterial EF‐Tu‐equivalent factor is also immunologically unrelated to eubacterial EF‐Tu and does not cross react with antibodies against Escherichia coli EF‐Tu. The pulvomycin and kirromycin reactions thus provide new phyletic markers for archaebacterial ancestry.