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The presence of alkali‐labile sites in DNA daughter chains of UV‐irradiated Escherichia coli
Author(s) -
Slezáriková Viera,
Sedliaková Milena
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80818-1
Subject(s) - escherichia coli , alkali metal , dna , chemistry , irradiation , centrifugation , sucrose , biochemistry , sucrose gradient , gene , organic chemistry , enzyme , physics , nuclear physics
DNA newly synthesized in UV irradiated Escherichia coli B/r Hcr + was 2 min pulse‐labeled at various periods, then denatured and analysed by sucrose gradient centrifugation either in neutral or in alkaline conditions. Data indicate that in DNA of damaged cells alkali‐labile sites are produced. In cells saturated with inducible proteins production of alkali‐labile sites disappears in ∼1 h. In the absence of inducible proteins production of alkali‐labile sites continues.