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Carbodiimide‐induced protein—RNA crosslinking in mammalian ribosomal subunits
Author(s) -
Buisson M.,
Reboud A.M.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80817-x
Subject(s) - carbodiimide , ribosome , rna , ribosomal rna , chemistry , ribosomal protein , biochemistry , microbiology and biotechnology , sedimentation coefficient , protein biosynthesis , protein subunit , biophysics , biology , enzyme , gene
RNA—protein interactions in the 60 S subunits of rat liver ribosomes were studied using 1‐ethyl‐3‐dimethylaminopropyl carbodiimide (EDC) under conditions which neither changed the sedimentation coefficient of subunits nor the intactness of their rRNA. EDC induced RNA—protein and protein—protein crosslinkings. Proteins crosslinked to 28 S RNA were identified by two‐dimensional gel electrophoreses as L17, L19, L23a and L37a, shown to react with 28 S RNA when using a low dose of UV radiation. Attempts have also been made to use EDC for the studies of RNA—protein interactions in 40 S ribosomal subunits.