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Virion‐associated RNA polymerase of bacteriophage Φ6 synthesizes three complete transcripts of double‐stranded RNA genome in vitro
Author(s) -
Emori Yasufumi,
Iba Hideo,
Okada Yoshimi
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80815-6
Subject(s) - rna , rna dependent rna polymerase , small nuclear rna , rna polymerase , polymerase , transcription (linguistics) , rna polymerase i , microbiology and biotechnology , rna editing , bacteriophage , biology , genetics , dna , gene , escherichia coli , linguistics , philosophy
Three single‐stranded RNA transcripts synthesized in vitro by a virion‐associated RNA polymerase of bacteriophage Φ6 were sequenced at their 5′‐ and 3′‐termini. The sequences agreed with those of the + strands of the 3 double‐stranded RNA segments [FEBS Lett. (1982) 141,111–115]. The results show that the transcription by Φ6 RNA polymerase initiates exactly at the 3′‐ends of the template RNAs (− strands of the genomic RNA) and terminates exactly at the 5′‐ends.