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Solution conformation of the biantennary N‐linked oligosaccharide of human serotransferrin using 1 H NMR nuclear Overhauser effect measurements
Author(s) -
Homans S.W.,
Dwek R.A.,
Fernandes D.L.,
Rademacher T.W.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80799-0
Subject(s) - nuclear overhauser effect , chemistry , oligosaccharide , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , stereochemistry , biochemistry , physics
The conformation in solution of the biantennary complex type oligosaccharide unit derived from human serotransferrin has been investigated using 1 H— 1 H Nuclear Overhauser Effect (NOE) measurements at 300 MHz. From quantitation of the NOE, the α(1–3) antenna is shown to exist in a preferred solution conformation with respect to the mannosyl‐chitobiose core. The flexibility of the α(1–6) arm, together with the absence of NOE data between this arm and the core, indicates that, in contrast to the α(1–3) arm the α(1–6) arm has no preferred conformation with respect to the core.