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Detection of glycogen in a glycogen storage disease by 13 C nuclear magnetic resonance
Author(s) -
Stevens Anthony N.,
Iles Richard A.,
Morris Peter G.,
Griffiths John R.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80796-5
Subject(s) - glycogen , glycogen storage disease , nuclear magnetic resonance , magnetic resonance imaging , chemistry , resonance (particle physics) , medicine , biochemistry , physics , atomic physics , radiology
The livers of gsd/gsd rats homozygous for the glycogen storage disease phosphorylase b kinase deficiency were observed by 13 C NMR using a surface coil. Clear signals were detected from glycogen. The concentration of glycogen as determined by NMR was ∼3‐times that found in normal strains agreeing well with chemical determinations Starvation did not significantly reduce the glycogen content of the livers with glycogen storage disease whereas it reduced the signal below detectability in normal rats. Difference spectra of starved normal rats from fed gsd/gsd rats gave spectra similar in appearance to that of purified glycogen. Glycogen both in vivo and in vitro is fully visible using 13 C NMR.