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Mechanism of chain initiation by dextransucrase
Author(s) -
Parnaik Veena K.,
Mayer Robert M.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80794-1
Subject(s) - dextransucrase , sucrose , chemistry , fructose , dextran , hydrolysis , biochemistry , substrate (aquarium) , acceptor , stereochemistry , leuconostoc mesenteroides , biology , bacteria , lactic acid , ecology , genetics , physics , condensed matter physics
Dextran was synthesized using dextransucrase from Streptococus sanguis 10558 and (F)‐[ 14 C]sucrose as substrate to test the possibility that sucrose may be the initial acceptor for glucose. If sucrose is the initial acceptor, then dextran chains should have [ 14 C] fructose in a terminal ‘sucrose’ linkage which can be cleaved under mild conditions. Although incorporation of [ 14 C]fructose into dextran was observed, the label was not released by mild hydrolysis, indicating that sucrose is not the initiator for dextran synthesis. Incorporation of [ 14 C]fructose into dextran might represent its ability to act as an acceptor, as suggested by the isolation of leucrose as a by‐product in the reaction.