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Non‐processive transcription of poly[d(A—T)] by wheat germ RNA polymerase II
Author(s) -
Durand Robert,
Job Claudette,
Teissère Marcel,
Job Dominique
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80793-x
Subject(s) - transcription (linguistics) , polymerase , microbiology and biotechnology , rna , rna polymerase , rna polymerase ii , oligonucleotide , rna polymerase i , rna dependent rna polymerase , chemistry , nucleotide , enzyme , biology , dna , biochemistry , gene expression , gene , promoter , philosophy , linguistics
RNA product distribution obtained during the transcription of poly[d(A—T)] by wheat germ RNA polymerase IIA under various experimental conditions was analyzed by high resolution polyacrylamide gel electrophoresis. Poly[r(A—U)] synthesis proceeded as if wheat germ RNA polymerase II was a non‐processive enzyme: a ladder of RNA products of increasing lengths was obtained, which apparently, terminated at every other nucleotide. RNA release was not dependent upon nucleoside triphosphate substrate concentrations. A likely explanation would be that ternary complexes enzyme: DNA: RNA were very much unstable; moreover, oligonucleotides released were not re‐used for further elongation by the enzyme.