Premium
Degradation of short‐lived proteins is decreased by centrifugation
Author(s) -
Knecht E.,
Hernández-Yago J.,
Grisolía S.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80792-8
Subject(s) - centrifugation , cytoplast , enucleation , protein degradation , protein biosynthesis , cycloheximide , messenger rna , cytochalasin b , chemistry , biology , microbiology and biotechnology , biochemistry , cytoplasm , in vitro , gene , genetics
We have examined the effects of enucleation and of inhibitors of mRNA synthesis (actinomycin D and cordycepin) on protein turnover of HeLa cells. Enucleation markedly inhibited the rate of protein degradation for short‐lived proteins. However, cells centrifuged in the absence of cytochalasin B at the speed required to obtain cytoplasts showed protein degradation rates identical to those of cytoplasts, while inhibitors of mRNA synthesis did not affect the process. Although enucleation may affect degradation of specific proteins, these results suggest that centrifugation is largely responsible for the inhibition of protein degradation in cytoplasts.