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Transferrin protein and iron uptake by cultured hepatocytes
Author(s) -
Sibille Jean-Claude,
Octave Jean-Noël,
Schneider Yves-Jacques,
Trouet André,
Crichton Robert R.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80769-2
Subject(s) - transferrin , endocytosis , transferrin receptor , extracellular , chemistry , incubation , biochemistry , receptor
The binding and uptake of 59 Fe‐loaded 3 H‐labelled rat transferrin by cultured rat hepatocytes was investigated. At 4°C, there is no evidence for a specific binding of transferrin which could be related to the association of neo‐synthesized transferrin with plasma membrane receptors. At 37°C, iron uptake is much more important than transferrin uptake; it proceeds linearly over the time of incubation, is largely proportional to the extracellular transferrin concentration, and is compatible with uptake by fluid phase endocytosis. The difference observed between iron and transferrin uptake implies the existence of a mechanism allowing the reutilization of transferrin after iron delivery.