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Phosphorylation of tropomyosin during development in mammalian striated muscle
Author(s) -
Heeley D.A.,
Moir A.J.G.,
Perry S.V.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80716-3
Subject(s) - tropomyosin , phosphorylation , cardiac muscle , skeletal muscle , gel electrophoresis , biology , myocyte , microbiology and biotechnology , polyacrylamide gel electrophoresis , alpha (finance) , biochemistry , chemistry , actin , anatomy , medicine , construct validity , nursing , patient satisfaction , enzyme
α‐Tropomyosin from rat cardiac muscle was shown by two‐dimensional gel electrophoresis to become phosphorylated when tissue slices were incubated in Eagle's medium supplemented with 32 P i . In the adult rat and mouse heart the level of phosphorylation was ∼30%, but the level was much higher in the foetal heart (60–70%). A similar developmental trend was observed in skeletal muscle from the rat and mouse, where phosphorylated forms of both α‐ and β‐tropomyosins were observed. When rat cardiac cells were grown in tissue culture in the presence of 32 P i , radioactivity was incorporated into the region of the gel containing tropomyosin.