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Monoclonal antibodies to choline acetyltransferase of rat brain
Author(s) -
Ichikawa Tomoyuki,
Ishida Isao,
Deguchi Takeo
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80626-1
Subject(s) - choline acetyltransferase , monoclonal antibody , acetyltransferase , antibody , enzyme , chemistry , subclass , microbiology and biotechnology , acetyltransferases , choline , biochemistry , monoclonal , biology , acetylation , cholinergic , immunology , endocrinology , gene
Monoclonal antibodies to rat brain choline acetyltransferase were produced by the hybridoma technique. Two stable cell lines, Ab‐57 and Ab‐60, secreted immunoglobulin of subclass IgG1. The monoclonal antibodies bound to choline acetyltransferase without blocking catalytic activity. Affinity of Ab‐57 was 100‐times higher than that of Ab‐60. Both antibodies bound to the rat enzyme in a mutually exclusive fashion. The antibodies showed cross‐species reactivity with choline acetyltransferase from several mammalian brains.