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The recognition of a soluble cytochrome P450 in rat liver
Author(s) -
Hasegawa Tohru
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80616-9
Subject(s) - hexobarbital , microsome , cytochrome p450 , aniline , substrate (aquarium) , chemistry , dithionite , cytochrome , biochemistry , chromatography , enzyme , organic chemistry , biology , ecology
The presence of hepatic soluble cytochrome P450 was investigated in the rats that were pretreated with CCl 4 , CS 2 and DENA + PCBs. The dithionite‐reduced CO difference spectrum of the hepatic 204000 × g supernatant of these rats showed its maximum at 455 nm. Aniline and hexobarbital could react with the hepatic 204000 × g supernatant to give the substrate binding spectra of P450; differences were observed between the substrate‐induced difference spectra of the 204000 × g supernatant and that of the microsomal fraction. These results indicate the existence of a hepatic soluble P450.